STEPdb: Sub-cellular Topologies of E.coli Polypeptides.

K-12(MG1655) BL21(DE3) Both EPEC (O127:H6)

Bacterial cell cartoon of sub-cellular locations

STEPdb database:

The STEPdb database contains a comprehensive characterization of sub-cellular localization and topology of the Escherichia coli proteome. Current annotation is a result a multi-combinatorial analysis including existing databases, predictions of bioinformatic tools and exhaustive literature survey and manual curation. Tools for sequence similarity searches, prediction of sub-cellular location, visualization of membrane proteins topologies and protein complexes are included.


For publication of results, please cite:
  • Proteome-wide sub-cellular Topologies of E. coli.
    Georgia Orfanoudaki and Anastassios Economou
    Mol Cell Proteomics 13:, 3674-3687, 2014

    doi: 10.1074/mcp.O114.041137

Relevant papers:
  • The Escherichia coli peripheral inner membrane proteome.
    Papanastasiou M, Orfanoudaki G, Koukaki M, Kountourakis N, Sardis MF, Aivaliotis M, Karamanou S, Economou A.
    Mol Cell Proteomics 12:, 599-610, 2013

    doi: 10.1074/mcp.M112.024711
    Supplemental Data: DC1

  • Rapid label-free quantitative analysis of the E. coli BL21(DE3) inner membrane proteome
    Papanastasiou M, Orfanoudaki G, Kountourakis N, Koukaki M, Sardis MF, Aivaliotis M, Tsolis KC, Karamanou S, Economou A.
    ​PROTEOMICS accepted, before proof

    Main Text: pdf
    Supplemental Data: pdf


 ©2015 e. Economou Lab